MR DNA offers comprehensive bacterial 16S rRNA sequencing services, providing unparalleled insights into bacterial communities and their roles in various environments. Using advanced techniques and optimized primers, our 16S sequencing enables precise microbial profiling across diverse ecosystems, from soil and water to human and animal microbiomes. With decades of experience and a commitment to excellence, MR DNA ensures high-quality data to drive impactful research and discoveries.
Beyond 16S sequencing, MR DNA specializes in a wide array of sequencing applications, including metagenomics, metatranscriptomics, fungal ITS sequencing, and long-read amplicon sequencing. Our expertise spans both Illumina and PacBio platforms, enabling us to tailor solutions to meet your project’s specific needs. Whether you’re delving into bacterial diversity, functional gene analysis, or comparative genomics, we have the technology and expertise to support your research.
With MR DNA, you’re partnering with a leader in sequencing and bioinformatics. From microbial profiling to complete metagenome assemblies, our solutions are designed to empower researchers with actionable data. Visit MR DNA to learn more about how we can support your sequencing needs and help you unlock the secrets of the microbial world.
16S rRNA sequencing identifies bacteria by targeting the 16S ribosomal RNA gene, a highly conserved region present in the genome of all bacteria. This gene contains both conserved and variable regions, making it ideal for distinguishing bacterial species.
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Conserved Regions as Universal Targets: The conserved regions of the 16S rRNA gene are nearly identical across all bacterial species. This universality allows the use of broad-spectrum primers to amplify the gene from a wide range of bacterial genomes in a single sample.
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Variable Regions for Identification: The 16S gene also has nine hypervariable regions (V1-V9) interspersed within its conserved framework. These variable regions contain sequences that differ significantly between bacterial species. By sequencing these regions, researchers can identify unique genetic signatures specific to individual bacterial taxa.
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Sequencing and Analysis: After amplification of the 16S rRNA gene using PCR, the DNA is sequenced using high-throughput platforms like Illumina or PacBio. The resulting sequence data is then compared against a reference database of known 16S sequences (e.g., SILVA, RDP, or Greengenes) to determine the taxonomy of the bacteria present.
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Microbial Community Profiling: 16S sequencing allows researchers to identify and quantify bacterial species in complex communities. This method is widely used in microbiome studies, environmental surveys, and clinical diagnostics to understand bacterial diversity, track changes in microbial populations, and discover previously unknown species.
By leveraging the power of 16S sequencing, researchers can uncover detailed insights into bacterial communities and their functional roles in various ecosystems.
Long-read HiFi sequencing for 16S rRNA gene analysis is a transformative approach offered by MR DNA, combining cutting-edge PacBio HiFi sequencing with expertise in microbiome research. This method enables the generation of high-accuracy, full-length 16S rRNA sequences, providing unparalleled resolution for bacterial identification and taxonomy.
Unlike traditional short-read sequencing, which often targets specific hypervariable regions (e.g., V3-V4), HiFi sequencing captures the entire 16S rRNA gene (approximately 1,500 base pairs). This comprehensive approach eliminates the biases and limitations associated with partial gene sequencing, allowing for more accurate differentiation of closely related species and detection of rare or novel taxa.
The benefits of long-read HiFi sequencing extend beyond taxonomic precision. The highly accurate reads (with >99.9% accuracy) ensure confidence in the results, even for complex or low-abundance taxa. Additionally, MR DNA’s bioinformatics expertise integrates state-of-the-art pipelines for seamless data analysis, leveraging curated databases for robust taxonomy assignments and functional insights.
Whether exploring microbial diversity in environmental samples, investigating health-associated microbiomes, or conducting industrial research, MR DNA’s long-read 16S sequencing services empower researchers with the most detailed and reliable microbial profiles available. Learn more and discover the future of microbiome analysis at MR DNA.